Biosynthesis of Gold Nanoparticles (AuNPs) by Diopatra claparedii Grube, 1878 (Polychaeta: Onuphidae) and Its Antibacterial Activity

Gold nanoparticles (AuNPs) have gained attention as it possesses outstanding physicochemical properties, and utilised in variety of applications especially biomedical pharmaceutical. Majorly, AuNPs are produced by conventional methods (chemical physical). However, these bring several drawbacks such toxic, hazardous, low yield non-environmental friendly. Hence, biosynthesis that compliance with ‘greener’ approach becomes vitals. In this study, marine tube worm Diopatra claparedii (polychaetes) was employed reducing agent the AuNPs. The biosynthesised D. extract successfully prepared under ambient temperature normal atmospheric conditions. formation AuNPS confirmed appearance surface Plasmon resonance (SPR) bands around 540 to 560 nm characterized UV-Vis spectroscopy. Scanning electron microscopy (SEM) showed mostly agglomerated spherical like shapes size ranging from 100 400 nm. Meanwhile, transmission (TEM) particles range 25 60 nm, also mainly form shape. particle a 50-100 dynamic light scattering (DLS). Fourier-transform infrared (FTIR) spectrum indicated existence functional groups. Biosynthesised gave inhibition on bacterial growth (Staphyloccus aureus, S. epidermidis, Escheratia coli, Salmonella typhi) through antibacterial assessment.